FIGURE 4: Effect of PQ-stress and aging on ROS-scavenging pathways.

(A) Transcriptome data of ROS scavenger enzymes. Colors and numbers indicate relative expression of transcripts of the indicated genes and treatment. Relative expression was calculated by dividing tpm of juvenile PQ-stressed samples by tpm of juvenile control (juv PQ), middle-aged PQ-stressed samples by middle-aged control (ma PQ), senescent PQ-stressed samples by senescent control (sen PQ) and senescent control by juvenile control (aging).

(B,C) The overall SOD activity and H2O2 decomposition rate were measured in protein extracts of juvenile (juv), middle-aged (ma) and senescent (sen) P. anserina culturesgrown in PQ-free medium or in medium supplemented with 200 µM PQ.

(B) Overall SOD activity is shown as mean relative expression to the juvenile wild type ± standard error. n = 6. Student’s t test p-values: juv to juv PQ pV = 0.049; juv to sen pV = 0.007.

(C) Besides in wild-type strains (control and PQ), measurement of H2O2 decomposition was carried out in a ΔPaCatB strain to estimate the portion of activity related to PaCATB. Data of wild type is shown as mean ± standard error. n = 9 – 11 for wt; n = 1 for ΔPaCatB. Student’s t-test p-values of wild type: juv to sen pV = 0.004; juv to juv PQ pV = 7.05E-5; ma to ma PQ pV = 2.41E-5; sen to sen PQ pV = 0.016.

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this. Please refer to our "privacy statement" and our "terms of use" for further information.