FIGURE 6: Effect of copper and PQ on lifespan and growth.

(A) Lifespan of P. anserina on M2 medium supplemented with 20 µM PQ (n = 20; median lifespan = 56.5 days; p < 0.001), 100 µM PQ (n = 20; median lifespan = 21.6 days; p < 0.001), 200 µM PQ PQ (n = 20; median lifespan = 3.5 days; p < 0.001), 100 µM CuSO₄ (n = 20; median lifespan = 21.5 days; p < 0.001) or 100 µM PQ and 100 µM CuSO₄ combined (n = 3.8; median lifespan = 3 days; p < 0.001). P-values were determined in comparison with untreated control (n = 20; median lifespan = 27 days) by 2-tailed Wilcoxen rank sum test.

(B) The growth distance of cultures from (A) was measured after 10 days on M2 medium. n = 20. P-Value against control or as indicated was determined by 2-tailed Wilcoxen rank sum test.

(C) Differential transcript abundance of the wild type grown under PQ-stress and in the grisea mutant grown without additional PQ is depicted in Venn diagrams of up- (left) or down- (right) regulated transcripts (factor < 3; pV < 0.01) during PQ-stress in juvenile P. anserina and in the mutant grisea.