FIGURE 1: Intrachromosomal gene conversion is increased in RNase H2-defective cells.

(A) Recombination rates of gene conversion are shown as median rates with 95% confidence limits (n = 18) in RNase H1 and RNase H2 mutant strains. The reporter is a duplication of leu2 genes at the LEU2 locus.

(B) Effect of RNase H1 over-expression in intrachoromosomal gene conversion rates. Recombination rates of the ade2 duplication reporter with a Gal-Rnh1 plasmid are shown with 95% confidence limits (n = 12).

(C) Effect of TOP1 deletion on the gene conversion recombination rate using the leu2 duplication reporter. The wild type (WT) and rnh202∆ rates are taken from panel A.

(D) Recombination rates of gene conversion are shown as median rates with 95% confidence limits (n = 18) in RNase H2 mutant strains using a different recombination reporter consisting of a duplication of ade2 genes at the ADE2 locus. The effect of TOP1 deletion on this reporter is also shown.

(E) Chromosome fragment loss in a haploid strain is shown. Median rates with standard deviations are displayed.