FIGURE 1: Absence of the AAC proteins promotes survival of S. cerevisiae to acetic acid and diamide reverting the sensitivity phenotype of Δpor1 cells.

(A) Survival of wt, Δaac1/2/3, Δpor1 and Δaac1/2/3Δpor1 cells after treatment with acetic acid (180 mM) was determined by c.f.u. counts after 60, 120 and 180 minutes of treatment, considering the total c.f.u. number at T0 as 100 % survival.

(B) Survival of wt, Δaac1/2/3, Δpor1 and Δaac1/2/3Δpor1 cells after treatment with diamide (16 mM) was determined by c.f.u. counts, as previously mentioned. Cells were pre-cultured in YPD, and then grown O.N. in YPGal or YPD until an O.D._640nm of 1.5-2.0 was reached. Data represent mean ± SEM of at least 3 independent experiments. Statistical analysis was performed using a Two-way ANOVA and Bonferroni post-tests (P-values: (*) P < 0.05; (**) P < 0.01; (***) P < 0.001).