FIGURE 1: Isc1p-deficient cells exhibit enhanced TORC1 activity in vivo. The TORC1-dependent C-terminal phosphorylation of Sch9p was used to assess TORC1 activity in vivo.

(A) S. cerevisiae BY4741 and isc1Δ cells transformed with pJU676 (expressing SCH9-5HA) were grown in SC-medium to the exponential phase and NTCB-treated protein extracts were analyzed by immunoblotting using anti-HA antibody, as described in Materials and Methods. A representative blot out of three is shown.

(B) Quantification of band intensities was performed by densitometry. The ratio between phosphorylated and unphosphorylated Sch9p (Pi+/Pi) is shown. Values are mean ± SD of at least three independent experiments. ****p<0.0001.