FIGURE 2: Overexpression of SIR2 and deletion of SUM1 have complementary roles in regulation of actin cytoskeletal integrity.

(A) Wild-type, sum1∆, SIR2 overexpressing (SIR2 o/e), and sum1SIR2 o/e cells were stained with rhodamine-phalloidin as described in Materials and Methods. Scale bar represents 1 µm. Outlines were drawn from brightfield images.

(B) Notched dot box plot of the number of actin cables in wild-type, sum1∆, SIR2 o/e, and sum1SIR2 o/e cells.

(C) Number of actin patches in the mother cell of wild-type, sum1∆, SIR2 o/e, and sum1SIR2 o/e cells. n = 56 – 69 cells per strain. Data is representative of 2 independent trials. ** = P < 0.01, *** = P < 0.001. P values were calculated using Kruskal-Wallis testing.

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this. Please refer to our "privacy statement" and our "terms of use" for further information.