FIGURE 3: Measurement of iron content.

(A) Iron sensitivity. Equal amounts of serial dilutions of cells from exponentially grown cultures (105, 104, 103, 102, 101) were spotted onto 40 medium without pantothenate supplemented with 5 mM and 7 mM FeS04. The growth was scored after 5 days of incubation at 28°C.

(B) Iron level. Cellular iron levels was quantified in cells grown up to early stationary phase in YNB glucose (0,6%) medium. **P < 0.01 (unpaired two-tailed t-test).

(C) PMS/DCPIP (phenazyne methosulfate/dichlorophenolindophenol) reductase and decylubiquinone reductase activities were measured in mitochondria extracted from cells grown exponentially at 28°C in mineral medium (40) plus 0.2% glucose and 2% galactose without pantothenate. The values of the COASY mutant are expressed as percentage of the activities obtained in the wild type strain.

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