FIGURE 4: Heme reduction strengthens ARTs’ anti-mitochondrial effect.

(A) ART had little negative effect on O2 consumption in both the WT and HEM2-KD strains (ns, no significance).

(B) Similar levels of mitochondrial depolarization at much lower ART concentrations could be observed when heme is down-regulated. The membrane potential ( Δym ) was assessed by measuring the Δym-dependent uptake of Rh123. Lower uptake of the probe indicates lower Δym.

(C, D) HEM2-KD yeast has reduced ART’s metabolism rate as compared to WT. (C) is the chromatogram of ART in LC-MS analysis. The peak of ART is at 6.94 min.

(D) is quantitative measurement of results from three parallel experiments. ***, p<0.001.

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this. Please refer to our "privacy statement" and our "terms of use" for further information.