FIGURE 5: Nup100 is specifically required for INO1 clustering during memory. Diploid cells having both alleles of INO1 marked with the LacO array and expressing GFP-LacI were grown under repressing, activating or memory conditions. Left: The distribution of distances between the two loci in ~ 100 cells, binned into 0.2 µm bins. P values were calculated using a Wilcoxon Rank Sum Test. Right: the fraction of cells in which the two loci were ≤ 0.55 µm. P values were calculated using a Fisher Exact Test.

(A and B) NUP100/NUP100 diploids.

(C and D) nup100∆/nup100∆ diploids.