FIGURE 6: Cox1 I¹⁰¹F is unable to restore respiratory growth of cox14∆ cells.

(A) COX14 was either deleted in a WT strain or in a cox23∆ suppressor strain. High-copy or low-copy plasmids containing COX23 were transformed to cox14∆ cox23∆ deletion strain to make the strain a cox14∆ containing COX1 suppressor mutation. The resulting cells were tested for respiratory growth.

(B) cox23∆ mutant cells with and without the Cox1 I¹⁰¹F allele were plated on glycerol/lactate medium containing increasing concentrations of paraquat (0 – 5 mM) at 30°C.