Back to article: Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways

FIGURE 1: LYS2-based frameshift reversion assays. (A) Cartoon illustrating how stop codons in alternative reading frames define a reversion window (blue). The horizontal arrow indicates the direction of transcription and asterisks correspond to frameshift mutations. A stop codon encountered during translation is red; a gray stop codon is in a different reading frame and does not affect translation. (B) Sequence of the region of the LYS2 ORF that contains the lys2ΔBgl reversion window (gray), which is delimited by the boxed stop codons. Mononucleotide runs >3N are underlined and the BglII site is highlighted yellow.

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this. Please refer to our "privacy statement" and our "terms of use" for further information.