Back to article: Type II-Metacaspases are involved in cell stress but not in cell death in the unicellular green alga Dunaliella tertiolecta
FIGURE 2. MCs immunodetection in D. tertiolecta exposed to PAR + UVA + UVB (PAB) for six days with a polyclonal antibody specific for MC9 (α-AtMC9) from the model plant A. thaliana (A); negative control with the 1ary antibody substituted by its correspondent pre-immune sera (B); positive cross-reaction controls consisting of proteins extracted from A. thaliana wild type leaves (Atwt), from A. thaliana over-expressing MC9 (Atoe9) and MC1 ( Atoe1 ) electrophoresed and western blotted together with D. tertiolecta samples (Dt r1 and r2) and commercial pure rubisco protein probed against α-AtMC9 (C); positive control consisting of α-AtMC9 blocked with MC9 recombinant protein (RCMC9) and probed against Atwt, Atoe9, Atoe1, Dt r1 and r2 and pure rubisco protein (D); negative control consisting of α-AtMC9 blocked with pure rubisco and probed against Atwt, Atoe9, Atoe1, Dt r1 and r2 and pure rubisco (E).