FIGURE 6: Improved assembly of InvG in mitochondria lacking Tom20. (A) Mitochondria were isolated from the indicated strains transformed with either an empty vector (Ø) or a plasmid encoding InvG-HA. Further treatment and analysis were done as described in the legend of Fig. 4A. (B) Crude mitochondria were obtained from wild type (WT), tom20Δ or a strain overexpressing TOM20 (Tom20↑) harbouring a plasmid encoding InvG-HA. Further treatment and analysis were as described in the legend of Fig. 4B. (C) Crude mitochondria were obtained from WT, tom70/71Δ or a strain overexpressing TOM70 (Tom70↑) harbouring a plasmid encoding InvG-HA. Further treatment and analysis were done as described in the legend of Fig. 4B. (D) Whole cell lysate was obtained from the indicated cells and was analysed by SDS-PAGE followed by immunodecoration with antibodies against the indicated proteins. The steady state levels of InvG-HA in at least three experiments for each strain were quantified. The signal of Bmh1 was taken as a loading control. Levels of InvG-HA in the corresponding wild type cells were set to 100%. The bar diagram shows the mean values± s.d. of at least three independent experiments. (*, P < 0.05; two tailed Student's t-test).