FIGURE 5: Effects of mTOR inhibition on relative expression of metabolic enzymes in Salmonella infected RAW264.7 macrophages. Expression of aconitase (A), isocitrate dehydrogenase (B), succinate dehydrogenase (C) and lactate dehydrogenase (D) mRNA relative to the house keeping gene was determined by qRT-PCR after infecting RAW264.7 cells with Salmonella (MOI of 10) for 24 hours and after pre-treatment with rapamycin (200 nM) or saline (control) 1 h prior to infection is shown. The numbers of intramacrophage bacteria was determined by gentamycin protection assay after 24 hours (E). For these experiments, cells were treated with FeCl₃/hepcidin (50 μM/1 μg/ml, respectively) or DFP (50 μM) for 6 h prior to infection and supplemented with rapamycin (200 nM) or saline one hour before infection. Representative data from three independent experiments performed with duplicates or triplicates are shown. Graphs show means ± SEM. Student's t-test was used. Exact p-values are indicated in the graphs.