Back to article: Guidelines for DNA recombination and repair studies: Mechanistic assays of DNA repair processes

FIGURE 12: FRET-based endonuclease assay. (A) A representative 384-well plate view kinetic measurement of the FRET-based MUS81-EME1 assay, with compounds tested in 10-concentrations dose response. (B) Graph shows the slope / reaction rate for assay controls. Kinetic readout (Excitation 535 nm / Emission 665 nm) for 8 minutes at linear phase was measured per assay plate and data was calculated as slope from a time series of 12 data points representing the rate of reaction. The assay was robust with Z' performance of 0.74 ± 0.04.

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