Back to article: Stable and destabilized GFP reporters to monitor calcineurin activity in Saccharomyces cerevisiae

FIGURE 2: Reporter protein stability assessed via immunoblotting.(A-C) Representative immunoblots (A) and corresponding densitometric quantification (B, C) of reporter protein levels in exponentially growing cells lacking Pmr1 equipped with either pAMS366-4xCDRE-lacZ, pAMS366-4xCDRE-GFP or pAMS366-4xCDRE-GFPPEST at indicated times points after addition of cycloheximide (CHX) to a final concentration of 100 mg/L. Blots were decorated with antibodies against β-galactosidase or GFP, respectively, and Pgk1 as loading control. Molecular weight (kDa) as estimated by Page Ruler Protein standard. Values represent fold to t = 0 min. Means ± SEM, n = 4.

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