Back to article: Histone H3E73Q and H4E53A mutations cause recombinogenic DNA damage

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FIGURE 2: Histone H3E73Q or H4E53A mutants increase DNA damage.(A) Analysis of the percentage of S/G2 cells containing Rad52-YFP foci in H3 wild-type (H3WT), H3E73Q (H3E73Q), H4 wild-type (H4WT) and H4E53A (H4E53A) strains transformed with pWJ1344. A representative image of a cell with a Rad52-YFP focus is shown (n = 3). (B) Accumulation and quantification of the immunofluorescence signal of P-H2A in H3 wild-type (H3WT), H3E73Q (H3E73Q), H4 wild-type (H4WT) and H4E53A (H4E53A) strains detected by fluorescence-based western blot (n = 3). A representative fluorescence-based western blot and the BY4741 strain treated with 0.05% MMS for 1.5 hours as a control (n = 2) is shown. Actin is shown as the loading control. Means and SEM are plotted in all panels. *p ≤ 0.05, ***p ≤ 0.001 (two-tailed Student's t-test).

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