Back to article: Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its first variants in fourplex real-time quantitative reverse transcription-PCR assays

FIGURE 2: Linear regression curves to assess synthetic RNA copy number in fourplex rRT-PCR assays. (A–G) For each representative graph, four primer-probe sets within one reaction mixture were used to detect serial 10-fold dilutions of four synthetic RNA transcripts ranging from 100 to 109 copies per reaction. The y and x axes of each graph are the Ct values and the log of the input of synthetic RNA transcripts. respectively. For each fourplex reaction, R2 indicates calculated linear coefficients and Eff. shows percentage of amplification efficiencies for each primer-probe set that is present with three other sets of primers and probes within one reaction master mixture. Fluorogenic probe color codes are as follows: blue, E Sarbeco; red, Orf1ab L; violet, N LSPQ; black, B2M; green, Spike 614G; orange, Orf1ab S; brown, Spike D614; pink, Spike 501Y; and gold, Spike N501. The graphs represent quantification of the results of three independent experiments.

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