Back to article: Aeration mitigates endoplasmic reticulum stress in Saccharomyces cerevisiae even without mitochondrial respiration

image description

FIGURE 1: HAC1-mRNA splicing is compromised by aerobic agitation.(A) Procedures for culturing and ER-stress induction of S. cerevisiae cells under the aerobically shaken condition or under the static condition. ER-stress agents (DTT, tunicamycin, or ethanol) were added to the media 4-hr after culture start. (B) Optical density of cultures (wild-type BY4742 cells) was monitored under non-stressing conditions. (C-E) Wild-type BY4742 cells were cultured and were ER-stressed (or remained non-stressed) as shown in panel A, and were checked for HAC1-mRNA splicing. n.s. (not significant): p > 0.05, *: p < 0.05, **: p < 0.01, ***: p < 0.001.

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this. Please refer to our "privacy statement" and our "terms of use" for further information.