Back to article: Cleavage-defective Topoisomerase I mutants sharply increase G-quadruplex-associated genomic instability

FIGURE 2: Deletion of WSS1 increases G4-induced recombination in Top1 catalytic mutant strains. (A, B) Recombination rates of WSS1 and wss1Δ yeast strains expressing indicated Top1 alleles. Rates are considered statistically significantly different if their 95% confidence intervals (shown as error bars) do not overlap [74]. (C) Top1-SUMO pull down experiment. All experiments were carried out in wss1Δ strains expressing C-terminally 3X-FLAG-tagged Top1 proteins and N-terminally 7XHis-tagged Smt3. SUMO-modified proteins were pulled down using Ni+ beads and Top1 proteins detected by western blotting with α-FLAG-HRP antibody. Top panel – inputs; bottom panel – pull down samples (PD). * denoted bands are mono- or poly-SUMOylated Top1 proteins.

74. Spell RM, Jinks-Robertson S (2004). Determination of mitotic recombination rates by fluctuation analysis in Saccharomyces cerevisiae. Methods Mol Biol 262: 3-12. 10.1385/1-59259-761-0:003

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