Back to article: Cellular cholesterol licenses Legionella pneumophila intracellular replication in macrophages


FIGURE 4: Lp uptake by U18666A- and ketoconazole-treated BMDMs. (a) Representative micrographs showing one completely (arrow head) and one partially internalized (arrow) bacterium. BMDMs were infected with GFP+ Lp ΔflaA and stained with anti-Lp antibody prior to permeabilization to distinguish intracellular (green) from surface-associated bacteria (red and green). (b) Phagocytosis of Lp by BMDMs pre-treated for 24 hrs with either DMSO, 3.3 µM ketoconazole or 5 µM U18666A prior to a two hr infection with GFP+ Lp ΔflaA. As a control, some BMDMs were treated with 5 µM cytochalasin D for the duration of the infection. Uptake index is calculated by normalizing the percentage of internalized bacteria for each condition to the percentage of internalized bacteria by the DMSO-treated cells in each experiment. Bars represent average of three biological replicates ± SD where > 100 bacteria for each treatment were scored. (n.s) not-significant; one-way ANOVA. (a-b) Representative data from one of two biological replicates are shown.

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this. Please refer to our "privacy statement" and our "terms of use" for further information.

Close