Back to article: A simple microfluidic platform to study age-dependent protein abundance and localization changes in Saccharomyces cerevisiae

Figure 2: Protein localization changes as cells age. MEP cells expressing Sir2-GFP (a), Hsp104-GFP (b), Hsp42-GFP (c), or Om45-GFP (d) were ana-lysed by time-lapse microscopy. Nup49-mCherry was used as marker of the nuclear periphery. Upper and lower images corresponding to the same division, usually 20-60 min apart, are shown to detect possible cell cycle-dependent changes in localization. Stacks of 8–9 sections (0.4 µm spacing) acquired at different time points were deconvolved (maximal projection is shown). Bright-field images were used to draw cell boundaries and count the number of divisions for the mother cells (age indicated with numbers). Representative images are shown. For Sir2-GFP, all six cells analysed showed the same phenotype. For Hsp104-GFP, Hsp42-GFP and Om45-GFP, 10 of 14, 6 of 7 and 11 of 13 cells, respectively, showed the phenotype depicted and described in the text. Scale bar, 2 µm.

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this. Please refer to our "privacy statement" and our "terms of use" for further information.