Back to article: Balanced CoQ6 biosynthesis is required for lifespan and mitophagy in yeast

FIGURE 1: CoQ6-dependent mitochondrial respiratory chain activities are negatively affected in yeast expressing Coq7 phosphomutant versions. Mitochondrial activities were measured with mitochondria purified from the coq7∆ strain harboring the indicated plasmids. pNMQ7 corresponds to the wild type COQ7 gene, pRS316 corresponds to the empty vector, pAAA and pDED correspond to the Coq7 phosphomutant versions and pmQ7 corresponds to a multicopy expression of wild type COQ7 gene.

(A) Top panel: CoQ6 quantification, bottom panel Western blots of mitochondrial samples probed with antibodies against Coq7. Mitochondrial activities: (B) NADH-DCIP reductase, (C) Complex II: succinate-DCIP reductase, (D) Complex III: Decylubiquinol-cytochrome c reductase, (E) NADH-cytochrome c reductase and (F) Complex II+III: Succinate-cytochrome c reductase. Results are expressed as nmol/mg mitochondrial protein.min. Data are mean ± SD, N ≥ 3 independent assays. ** P ≤ 0.001 compared to positive control samples.

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