Back to article: Farnesol inhibits translation to limit growth and filamentation in C. albicans and S. cerevisiae


FIGURE 4: Farnesol does not induce GCN4 expression or effect the dynamics of the eIF2B body. (A) and (B) CAI4 and gcn2Δ strains bearing the GCRE-Luc (A) and GCN4-Luc (B) reporters were treated with 1% (v/v) butanol or 100 µM farnesol for 2 h, extracts were prepared, then renilla luciferase activity was measured relative to untreated. Error bars = ± SEM. (C) Images from time-lapse microscopy studies using an eIF2Bγ-GFP expressing C. albicans strain. The strains were incubated in media with 1% butanol, or 100 µM farnesol, or they were left untreated (UT) for 15 min as indicated. Each row contains three stills from a series of 25 images over a period of 2 min, as well as a merged image of all 25 stills, which serves to depict the total extent of 2B body movement. (D) Bar chart depicting the mean distance moved in μ over a 2-min period from 24 time-lapse experiments. Error bars, ±1 SEM.

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this. Please refer to our "privacy statement" and our "terms of use" for further information.

Close