FIGURE 1: GFP-Opi1p re-localises to lipid droplets in cells lacking Scs3p. (A) GFP-Opi1p was expressed in wild-type cells (left) and scs3∆ cells (right) that were grown without inositol. In wild-type cells, GFP-Opi1p localises most prominently to the nuclear envelope, and also to the cortical ER that forms patches adjacent to the cell periphery [30]. In scs3∆ cells, GFP-Opi1p is only faintly on the nuclear envelope, and mostly in puncta. Arrowheads indicate these puncta and the corresponding positions in the accompanying transmission image. (B) scs3∆ cells expressing GFP-Opi1p as in A (1^st panel), and co-expressing RFP-Erg6p, a lipid droplet marker (2^nd panel), with pseudo-coloured green and red merge (panel 3, superposition = yellow) and transmission image (panel 4).

30. Loewen CJ, Gaspar ML, Jesch SA, Delon C, Ktistakis NT, Henry SA, Levine TP (2004). Phospholipid me-tabolism regulated by a transcription factor sensing phosphatidic acid. Science 304(5677): 1644-1647. http://dx.doi.org/10.1126/science.1096083