Figure 2 Ftr1 and Zrt1 function as micronutrient transceptors

Back to article: Identification of Ftr1 and Zrt1 as iron and zinc micronutrient transceptors for activation of the PKA pathway in Saccharomyces cerevisiae

FIGURE 2: Expression level of FTR1 and ZRT1 and cellular localization of Ftr1 and Zrt1 upon iron or zinc deprivation and re-addition.

Relative expression of FTR1 (A) and ZRT1 (B) in response to deprivation of iron or zinc, respectively, n = 2. As listed in Materials & Methods, a specific set of reference genes was used depending on the experiment. Localization of C-terminally tagged Ftr1-GFP (C) and Zrt1-GFP (D) under their substrate deprivation conditions (left panel) as well as after re-addition of 100 μM of the missing metal ion for 240 min (right panel), n = 2. Relative expression of FTR1 (E) and ZRT1 (F) at different time points after re-addition of 100 μM of the missing metal ion, iron or zinc, respectively, n = 2.