Back to article: Importance of polyphosphate in the Leishmania life cycle

FIGURE 3: PolyP and VTC4 fluctuation during promastigote growth of L. major and L. mexicana. L. major (A) and L. mexicana (C) promastigote cell concentrations were counted in independent cultures. (B, D) PolyP was extracted from cell lines, digested with polyphosphatase and Pi were quantified colorimetrically using malachite green. Pi concentrations represented polyP content of 3 x 107 cells for L. major (B) and 8 x 106 cells for L. mexicana (D). Results of a pool of minimum 3 independent experiments were expressed as mean ± SD. (E) Western blot analysis of 20 µg of L. major promastigotes pellet protein using the anti-cd-LmjVTC4 antibody and anti-α-tubulin as loading control.

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