Back to article: Improvement of biochemical methods of polyP quantification

FIGURE 1: High variability on polyP determination in S. cerevisiae.

(A) polyP content in mM concentration according to different authors. Values were converted to mM according to the following assumptions: i) The volume of the haploid cell is 42 fl, ii) One OD660 unit equals to 2.6 x 107 cells and represents 50 µg of RNA. The Mw for the [-PO3-] monomer is 79 g/mol.

(B) Flow chart of this work, showing the different possibilities explored in each of the three parts needed for determining polyPs: extraction, purification and quantification.

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