Back to article: Mutational analysis of fructose-1,6-bis-phosphatase FBP1 indicates partially independent functions in gluconeogenesis and sensitivity to genotoxic stress

FIGURE 2: Immuno-detection of the over-expressed Fbp1p mutants.

(A) Western-blot showing the levels of the over-expressed mutants in both cytosolic and nuclear fractions. Over-expression was verified in absence of endogenous Fbp1p expression in fbp1∆ strains. PGK1 and NOP1 were utilized as cytosolic and nuclear loading controls, respectively.

(B) Quantification of the western-blot signals, each band was normalized to the intensity of its loading control, resulting values where then normalized to those of the wt.

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