FIGURE 5: Characterization of the telomere C-strand 5’ end nucleotide in wild type U. maydis. (A) (Top) DNA from Wild type U. maydis was ligated separately to each of six individual telorette oligos, amplified using the UT4/5-F and teltail primers, and then subjected to Southern analysis. (Bottom) Wild type U. maydis DNA was ligated separately to each of six individual telorette oligos, amplified using the UT6-F and teltail primers, and then subjected to Southern analysis. (B) The number of UT4/5 and UT6 STELA products generated by each telorette oligos was determined and plotted as the percentage of total STELA products. (C) The position of the 5’ nucleotide identified by each individual telorette oligos is illustrated. Also indicated are the predominant U. maydis and mammalian 5’-end nucleotides.