Back to article: Temporal analysis of the autophagic and apoptotic phenotypes in Leishmania parasites

FIGURE 4: DNA degradation during Leishmania cell death and autophagy. (A) Microscopical observation of cells treated for 24 h with 0.1 µM of staurosporine and stained with DAPI and TUNEL (bar = 5 µm). TUNEL-positive nuclei indicate cells with a degraded nuclear DNA. (B) Percentage of TUNEL-positive cells in different cell culture conditions. A minimum of 1800 cells were counted from a minimum of four independent experiments. (C) Maximal kinetoplast fluorescence of cells in different culture conditions. A minimum of 200 cells were counted from minimum three independent experiments. Unpaired t-test, * p < 0.05, ** p < 0.01, *** p < 0.001.

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this. Please refer to our "privacy statement" and our "terms of use" for further information.