Back to article: Temporal analysis of the autophagic and apoptotic phenotypes in Leishmania parasites

FIGURE 6: When cultivated in PBS, cells first enter autophagy and then die when nutrients are lacking. (A) Percentage of autophagosome-containing cells (punctuated ATG8) and TUNEL-positive cells during culture of cells in PBS for 24 h (≥ 1128 cells from a minimum of three independent experiments for the ATG8 assay and from a minimum of five independent experiments for the TUNEL assay). Unpaired t-test: ns not significant, * p<0.05, ** p<0.01. (B) Real time microscopy of a representative GFP-ATG8 cell cultivated in PBS, showing ATG8 punctuated staining disappearance, nucleus and kinetoplast DAPI staining appearance concomitant with cell rounding up, then kinetoplast fluorescence decrease (bar = 5µm). (C) Sequence of events observed in the cell represented in (B), showing the death (characterized by DAPI permeability, cell rounding up and low kinetoplast fluorescence) of an autophagic cell (with GFP-ATG8 puncta). (D) Microscopical images of a cell in control conditions (cell cultivated in medium and not in PBS) representative of all the cells observed in the same conditions as in (B), for 300 min (bar = 5 µm).

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this. Please refer to our "privacy statement" and our "terms of use" for further information.