TABLE 1. IC50 of selected lysosomotropic drugs in T. brucei.

The inhibitory concentration (IC50) values of 6 lysosomotropic drugs on bloodstream form T. brucei were measured in this study and compared with those reported in mammalian cells (13, 42, 43). *Note that mammalian IC50 values for CQ, CPZ, PMZ, DSP and 4HT were measured on H9c2 rat-cardiomyocyte-derived cells, whereas mammalian IC50 for LeuLeu-OMe was estimated as the concentration observed to induce necrosis in 8 human cell lines including human neuroblastoma cell line SH-SY5Y, human immortalized keratinocytes HaCaT, human adenocarcinoma cell line HeLa, human hepatoma cell line HepG2, human colon carcinoma cell line CaCo-2, and human embryonic kidney fibroblasts HEK293, human breast carcinoma cell line MCF-7 and normal human dermal fibroblasts, NHDF, cytotoxic T lymphocytes and NK cells. LeuLeu-OMe = L-leucyl-L-leucyl methyl ester; CQ = Chloroquine; CPZ = Chlorpromazine; PMZ = Promethazine; DSP = Desipramine; 4HT = 4-hydroxytamoxifen. The IC50 values for T. brucei were shown as mean (95% confidence interval) from >=4 experiments for each drug.

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