TABLE 1. G1 parameters in different nutrients of wild type and tda1/tda1∆ cellsa.

a The strains were in the homozygous diploid BY4743 background. They were examined in at least 3 independent experiments, and in each experiment a technical duplicate was evaluated. The cells were cultured in 1% w/v yeast extract, 2% w/v peptone and the indicated amount of the carbon source shown in each case.

b Birth size was calculated from the size distributions of exponentially proliferating asynchronous populations, as described previously [15]. The average of at least three independent measurements, with a technical duplicate for each measurement, and the associated standard deviation are shown in each case.

c The specific rate of size increase (k) and critical size were calculated from elutriated synchronous cultures as we described previously [17], assuming an exponential mode of growth. The average of at least three independent experiments and the associated standard deviation are shown in each case.

d These are G1 estimates from the formula: G1(hours)=Ln(Critical size/Birth size)/k. Note that these values reflect the G1 length of newborn daughter cells. For G1 length calculations, the errors (± sd) were not propagated.

15. Truong SK, McCormick RF, Polymenis M (2013). Genetic Determinants of Cell Size at Birth and Their Impact on Cell Cycle Progression in Saccharomyces cerevisiae. G3 3(9): 1525-1530.

17. Hoose SA, Rawlings JA, Kelly MM, Leitch MC, Ababneh QO, Robles JP, Taylor D, Hoover EM, Hailu B, McEnery KA, Downing SS, Kaushal D, Chen Y, Rife A, Brahmbhatt KA, Smith R, 3rd, Polymenis M (2012). A systematic analysis of cell cycle regulators in yeast reveals that most factors act independently of cell size to control initiation of division. PLoS genetics 8(3): e1002590.

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