Back to article: Unresolved mystery of cyclic nucleotide second messengers, periplasmic acid phosphatases and bacterial natural competence
FIGURE 3: AphAHi activity is stimulated by cCMP and cUMP and inhibited by cAMP and cGMP. (A, B) Lineweaver-Burk plot of H. influenzae AphAHi kinetics in the presence of varied concentration of cAMP (A) and cGMP (B). (C, D) Michaelis-Menten plots of H. influenzae AphAHi in the absence and presence of cCMP (C), and cUMP (D). pNPP was used as the substrate and formation of the yellow p-nitrophenol was quantified spectrophotometrically at 405 nm. Data represent two biological replicates, each with two technical replicates.