FIGURE 2: S. enterica ridA mutants have a fitness defect in minimal medium. (A) Growth of S. enterica wild type (black) and a ridA mutant (red) in nutrient broth (NB, circles) and minimal glucose (11 mM) medium (triangles). (B) Competitive fitness of a S. enterica ridA mutant (ridA3::MudJ) grown in competition with wild type S. enterica in nutrient or minimal medium. The underlined genotype indicates the strain for which the competitive indices are shown in the corresponding graph. An asterisk (*) indicates a significant difference (p ≤ 0.05 as determined by Sidak’s multiple comparisons test) in CI values compared to control experiments in which the ridA mutant was grown in competition with a second ridA mutant (ridA4::Cm) in the relevant medium. Data shown are the mean and standard deviation of three independent biological replicates.