FIGURE 2: Production and secretion of recombinant RBD fusion proteins. (A) Schematic overview and expected mass of the secreted RBD fusion proteins. (B) Comparative Western blot analysis with antibodies against the RBD domain (left) and the GST domain (right) of RBD fusion proteins in the cell-containing pellet fraction (P) and supernatant fraction (S) following tetracycline induction of the according L. tarentolae liquid cultures. Recombinant RBD served as positive control (p.c.) and an induced culture without plasmid as negative control (n.c.). The calculated masses from panel A are indicated.