Proline metabolism regulates replicative lifespan in the yeast Saccharomyces cerevisiae

Authors:

Yukio Mukai1, Yuka Kamei1, Xu Liu1, Shan Jiang1, Yukiko Sugimoto2, Noreen Suliani binti Mat Nanyan2, Daisuke Watanabe2 and Hiroshi Takagi2

doi: 10.15698/mic2019.10.694
Volume 6, pp. 482 to 490, published 24/09/2019.

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Affiliations:

1 Department of Frontier Bioscience, Faculty of Bioscience, Nagahama Institute of Bio-Science and Technology, 1266 Tamura, Nagahama, Shiga 526-0829, Japan.

2 Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.

Keywords: 

proline, replicative lifespan, stress response, amino acid metabolism, yeast.

Corresponding Author(s):

Yukio Mukai, Department of Frontier Bioscience, Faculty of Bioscience, Nagahama Institute of Bio-Science and Technology, 1266 Tamura, Nagahama, Shiga 526-0829, Japan; Tel.: +81-749-64-8163; Fax: +81-749-64-8140; y_mukai@nagahama-i-bio.ac.jp Hiroshi Takagi, Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan; Tel.: +81-743-72-5420; Fax: +81-743-72-5429; hiro@bs.naist.jp

Conflict of interest statement:

The authors declare no conflict of interest.

Please cite this article as:

Yukio Mukai, Yuka Kamei, Xu Liu, Shan Jiang, Yukiko Sugimoto, Noreen Suliani binti Mat Nanyan, Daisuke Watanabe and Hiroshi Takagi (2019). Proline metabolism regulates replicative lifespan in the yeast Saccharomyces cerevisiae. Microbial Cell 6(10): 482-490. doi: 10.15698/mic2019.10.694

© 2019 Mukai et al. This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduc-tion in any medium, provided the original author and source are acknowledged.

Abstract:

In many plants and microorganisms, intracellular proline has a protective role against various stresses, including heat-shock, oxidation and osmolarity. Environmental stresses induce cellular senescence in a variety of eukaryotes. Here we showed that intracellular proline regulates the replicative lifespan in the budding yeast Saccharomyces cerevisiae. Deletion of the proline oxidase gene PUT1 and expression of the γ-glutamate kinase mutant gene PRO1-I150T that is less sensitive to feedback inhibition accumulated proline and extended the replicative lifespan of yeast cells. Inversely, disruption of the proline biosynthetic genes PRO1, PRO2, and CAR2 decreased stationary proline level and shortened the lifespan of yeast cells. Quadruple disruption of the proline transporter genes unexpectedly did not change intracellular proline levels and replicative lifespan. Overexpression of the stress-responsive transcription activator gene MSN2 reduced intracellular proline levels by inducing the expression of PUT1, resulting in a short lifespan. Thus, the intracellular proline levels at stationary phase was positively correlated with the replicative lifespan. Furthermore, multivariate analysis of amino acids in yeast mutants deficient in proline metabolism showed characteristic metabolic profiles coincident with longevity: acidic and basic amino acids and branched-chain amino acids positively contributed to the replicative lifespan. These results allude to proline metabolism having a physiological role in maintaining the lifespan of yeast cells.