A novel c-di-GMP signal system regulates biofilm formation in Pseudomonas aeruginosa
Authors:Gukui Chen1 and Haihua Liang1
doi: 10.15698/mic2020.06.720
Volume 7, pp. 160 to 161, published 23/04/2020.
1 Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi’an, ShaanXi, 710069, China.
Keywords:
c-di-GMP, SiaD, Biofilm, Pseudomonas aeruginosa
Corresponding Author(s):
Conflict of interest statement:
The authors declare no conflict of interest.
Please cite this article as:
Gukui Chen and Haihua Liang (2020). A novel c-di-GMP signal system regulates biofilm formation in Pseudomonas aeruginosa. Microbial Cell 7(6): 160-161. doi: 10.15698/mic2020.06.720
© 2020 Chen and Liang. This is an open-access article released under the terms of the Creative Commons At-tribution (CC BY) license, which allows the unrestricted use, distribution, and reproduction in any medium, pro-vided the original author and source are acknowledged.
Abstract:
The bacterial second messenger cyclic-di-GMP (c-di-GMP) controls biofilm formation and other phenotypes relevant to pathogenesis. The human pathogen Pseudomonas aeruginosa encodes 17 diguanylate cyclase (DGCs) proteins which are required for c-di-GMP synthesis. Therefore, the c-di-GMP regulatory system in P. aeruginosa is highly sophisticated. SiaD, one of the DGC enzymes, is co-transcribed with SiaA/B/C and has been shown to be essential for bacterial aggregate formation in response to environmental stress. However, the detailed function of this operon remains unknown. In our recent paper (Chen et al., doi: 10.15252/embj.2019103412), we have demonstrated that the siaABCD operon encodes a signaling network that regulates biofilm and aggregate formation by modulating the enzymatic activity of SiaD. Among this signaling system, SiaC interaction with SiaD promotes the diguanylate cyclase activity of SiaD and subsequently facilities the intracellular c-di-GMP synthesis; SiaB is a unique protein kinase that phosphorylates SiaC, whereas SiaA phosphatase can dephosphorylate SiaC. The phosphorylation state of SiaC is critical for its interaction with SiaD, which will switch on or off the DGC activity of SiaD. This report unveils a novel signaling system that controls biofilm formation, which may provide a potential target for developing antimicrobial drugs.