Novobiocin inhibits membrane synthesis and vacuole formation of Enterococcus faecalis protoplasts

Authors:

Rintaro Tsuchikado1,#, Satoshi Kami1,#, Sawako Takahashi1 and Hiromi Nishida1
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Affiliations:

1 Department of Biotechnology, Toyama Prefectural University, Japan.

Keywords: 

bacterial protoplasts/spheroplasts, DNA replication, Enterococcus faecalis, novobiocin, plasma membrane synthesis, vacuole formation

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Corresponding Author(s):

Hiromi Nishida, PhD, Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, Japan; Phone: +81-766-56-7500; Fax: +81-766-56-2498; hnishida@pu-toyama.ac.jp

Conflict of interest statement:

The authors declare to have no conflict of interest.

Please cite this article as:

Rintaro Tsuchikado, Satoshi Kami, Sawako Takahashi and Hiromi Nishida (2020). Novobiocin inhibits membrane synthesis and vacuole formation of Enterococcus faecalis protoplasts. Microbial Cell 7(11): 300-308. doi: 10.15698/mic2020.11.735

© 2020 Tsuchikado et al. This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduc-tion in any medium, provided the original author and source are acknowledged.

Abstract:

We demonstrate that plasma membrane biosynthesis and vacuole formation require DNA replication in Enterococcus faecalis protoplasts. The replication inhibitor novobiocin inhibited not only DNA replication but also cell enlargement (plasma membrane biosynthesis) and vacuole formation during the enlargement of the E. faecalis protoplasts. After novobiocin treatment prior to vacuole formation, the cell size of E. faecalis protoplasts was limited to 6 μm in diameter and the cells lacked vacuoles. When novobiocin was added after vacuole formation, E. faecalis protoplasts grew with vacuole enlargement; after novobiocin removal, protoplasts were enlarged again. Although cell size distribution of the protoplasts was similar following the 24 h and 48 h novobiocin treatments, after 72 h of novobiocin treatment there was a greater number of smaller sized protoplasts, suggesting that extended novobiocin treatment may inhibit the re-enlargement of E. faecalis protoplasts after novobiocin removal. Our findings demonstrate that novobiocin can control the enlargement of E. faecalis protoplasts due to inhibition of DNA replication.

doi: 10.15698/mic2020.11.735
Volume 7, pp. 300 to 308, published 10/08/2020.