FIGURE 3: Glycine and thiamine alleviate fitness defects of S. enterica ridA mutants. (A) Growth of S. enterica wild type (black) and a ridA mutant (red) in minimal glucose (11 mM) medium supplement with nothing (circles), 1 mM L-isoleucine (triangles) or 1 mM glycine and 100 nM thiamine (diamonds). (B) Competitive fitness of a S. enterica ridA mutant (ridA3::MudJ) grown in competition with wild type S. enterica in minimal glucose medium with indicated supplements. An asterisk (*) indicates a significant difference (p ≤ 0.05 as determined by Sidak’s multiple comparisons test) in CI values compared to control experiments in which the ridA mutant was grown in competition with a second ridA mutant (ridA4::Cm) in the indicated medium. Data shown are the mean and standard deviation of three independent biological replicates.