FIGURE 2: Hsp90 granules co-localize with conventional SGs upon heat stress but not upon arsenite stress. Co-localization analysis of Hsp90-GFP (green) and various SG markers tagged with tdTomato (red) after 2.0 mM arsenite treatment for 180 min at 27^◦C (A) or 42^◦C HS for 30 min (B). Arrows and arrowheads indicate representative Hsp90 granules and conventional SGs, respectively. The dashed arrows in the merged images correspond to those below the intensity plot. Scale bars: 10 µm. Co-localization of eIF4G and Pabp is shown in Fig. S3.