FIGURE 6: Geldanamycin decreases Hsp90/Nrd1 granules and increases the arsenite sensitivity. (A) Cells expressing Hsp90-GFP (green) and Nrd1- tdTomato (red) were pre-incubated with or without 10.0 µM GA for 20 hr and then treated with 2.0 mM arsenite for 150 min at 27^◦C. Scale bar: 10 µm. (B) Percentage of Hsp90 or Nrd1 granule positive cells. The graphs show mean ± SE (n=3). GA; geldanamycin. P*<0.05, P**<0.01; n. s., not significant; significantly different from each sample by paired Student’s t-test. (C) Effect of GA on the arsenite sensitivity. Cells were grown in the EMM to mid-log phase with 0.56% DMSO or 10.0 µM GA and adjusted to OD660 = 0.5. The cells were then diluted 300-fold with the fresh EMM containing DMSO or 10.0 µM GA and incubated in a 96-well plate with the indicated final concentrations of arsenite for 4 days at 27^◦C.