FIGURE 5: Effect of DNA methylation inhibitor and TET1 gene knockdown on PD-L1 gene expression in THP-1 cells with (A, B) pre-treatment of 5’AZA, and (C) shRNA-mediated TET1 knockdown. THP-1 cells were treated with H37Rv whole cell lysate (10 µg/ml) and EsxA protein (2 µg/ml). A) Production of PD-L1 protein was evaluated using a western blot assay with densitogram. Data are presented as the mean ± standard deviation (SD) for each group. PD-L1, programmed death-ligand 1. *: p < 0.05; **: p < 0.01; ***: p < 0.001.