Back to article: Microwave-assisted preparation of yeast cells for ultrastructural analysis by electron microscopy
FIGURE 2:Workflow for microwave-assisted permanganate fixation of yeast. (A)Microwave processing setup for glutaraldehyde and KMnO4 fixation with ColdSpot Pro temperature control device and temperature probe inserted in water-filed dummy. Tube.(B)Consecutive washing steps after KMnO4 fixation.(C)Recovery and cutting of the sample using a single-edge razor blade.(D)Side view and top view of flow through basket (PELCO Prep-Eze 12 well specimen holder) loaded with agar blocs placed in a 5 cm Petri dish. (E) Microwave vacuum chamber with temperature probe inserted in top plate (lid removed, visible in the front right).(F)Contrast enhancement using a modified Hiraoka grid staining kit.