Back to article: Microwave-assisted preparation of yeast cells for ultrastructural analysis by electron microscopy
FIGURE 3:Comparison of samples prepared by microwave-assisted permanganate fixation with and without glutaraldehyde fixation and metaperiodate treatment. (A) Wild type yeast cells were prepared as in Fig. 1E according to protocols 2 and 3.(B)Cells were prepared as in (A) without sodium metaperiodate (NaIO4) treatment.(C)Cells were prepared as in (A) without glutaraldehyde (GA) fixation.(D)Cell were prepared as in (A) without glutaraldehyde fixation and without metaperiodate treatment. All samples were prepared in parallel and cells are shown at the same magnification. CW, cell wall; ER, endoplasmic reticulum; M, mitochondrion; N, nucleus; V, vacuole. Bar, 1 µm.