The replication timing program in the hands of two HDACs

Authors:

Kazumasa Yoshida1,2, Armelle Lengronne1 and Philippe Pasero1

doi: 10.15698/mic2014.08.163
Volume 1, pp. 273 to 275, published 25/07/2014.

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Affiliations:

1 Equipe Labellisée Ligue Contre le Cancer, Institute of Human Genetics, UPR 1142, CNRS, 141 rue de la Cardonille, 34396 Montpellier, France.

2 Department of Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.

Keywords: 

DNA replication, epigenetics, histone deacetylases, budding yeast.

Corresponding Author(s):

Philippe Pasero, Equipe Labellisée Ligue Contre le Cancer, Institute of Human Genetics, UPR 1142, CNRS, 141 rue de la Cardonille, 34396 Montpellier, France ppasero@igh.cnrs.fr

Conflict of interest statement:

None.

Please cite this article as:

Kazumasa Yoshida, Armelle Lengronne and Philippe Pasero (2014). The replication timing program in the hands of two HDACs. Microbial Cell 1(8): 273-275.

© 2014 Yoshida et al. This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduc-tion in any medium, provided the original author and source are acknowledged.

Abstract:

In eukaryotes, duplication of genomic information depends on the sequential activation of multiple replication origins distributed along the chromosomes. Replication origins differ in initiation time, chromatin structure and three-dimensional position in the nucleus. Recently, we have performed a systematic analysis of the role of histone deacetylases (HDACs) in the regulation of origin activity in budding yeast. We have found that the epigenetic regulation of repetitive sequences is a key determinant of the DNA replication program. Indeed, our study revealed that two histone deacetylases, Rpd3 and Sir2, have opposite effects on the replication timing program. Rpd3 delays initiation at late origins, whereas Sir2 promotes efficient activation of early origins. Remarkably, we also found that Rpd3 and Sir2 regulate initiation at ~200 replication origins located within the ribosomal DNA (rDNA) array. We propose that this epigenetic regulation of repetitive origins controls the replication timing program by modulating the availability of limiting initiation factors.