Mitochondria-Associated Membranes (MAMs) are involved in Bax mitochondrial localization and cytochrome c release

Authors:

Alexandre Légiot1, Claire Céré1, Thibaud Dupoiron1, Mohamed Kaabouni1, Nadine Camougrand1 and Stéphen Manon1

doi: 10.15698/mic2019.05.678
Volume 6, pp. 257 to 266, published 15/03/2019.

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Affiliations:

1 Institut de Biochimie et de Génétique Cellulaires, UMR 5095 CNRS & Université de Bordeaux, Campus Carreire, CS61390, 1 Rue Camille Saint-Saëns, 33077 Bordeaux, France.

Keywords: 

Bax, apoptosis, mitochondria associated membranes, mitochondria, cytochrome c, ERMES, density gradients, Saccharomyces cerevisiae.

Corresponding Author(s):

Stéphen Manon, Institut de Biochimie et de Génétique Cellulaires, UMR 5095 CNRS & Université de Bordeaux, Campus Carreire, CS61390, 1 Rue Camille Saint-Saëns, 33077 Bordeaux, France; manon@ibgc.cnrs.fr

Conflict of interest statement:

The authors declare no conflict of interest.

Please cite this article as:

Alexandre Légiot, Claire Céré, Thibaud Dupoiron, Mohamed Kaabouni, Nadine Camougrand and Stéphen Manon (2019). Mitochondria-Associated Membranes (MAMs) are involved in Bax mitochondrial localization and cytochrome c release. Microbial Cell 6(4): 257-266. doi: 10.15698/mic2019.05.678

© 2019 Légiot et al. This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduc-tion in any medium, provided the original author and source are acknowledged.

Abstract:

The distribution of the pro-apoptotic protein Bax in the outer mitochondrial membrane (OMM) is a central point of regulation of apoptosis. It is now widely recognized that parts of the endoplasmic reticulum (ER) are closely associated to the OMM, and are actively involved in different signaling processes. We addressed a possible role of these domains, called Mitochondria-Associated Membranes (MAMs) in Bax localization and function, by expressing the human protein in a yeast mutant deleted of MDM34, a ERMES (ER-Mitochondria Encounter Structure) component. By affecting MAMs stability, the deletion of MDM34 altered Bax mitochondrial localization, and decreased its capacity to release cytochrome c. Furthermore, the deletion of MDM34 decreased the size of an incompletely released, MAMs-associated pool of cytochrome c.