Histone H3E73Q and H4E53A mutations cause recombinogenic DNA damage
Authors:Pedro Ortega1, Desiré García-Pichardo1, Marta San Martin-Alonso1, Ana G. Rondón1, Belén Gómez-González1 and Andrés Aguilera1
doi: 10.15698/mic2020.07.723
Volume 7, pp. 190 to 198, published 24/04/2020.
1 Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER), Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Seville, Spain.
Keywords:
chromatin, DNA replication, recombination, histone mutants
Corresponding Author(s):
Conflict of interest statement:
The authors declare no competing interests.
Please cite this article as:
Pedro Ortega, Desiré García-Pichardo, Marta San Martin-Alonso, Ana G. Rondón, Belén Gómez-González and Andrés Aguilera (2020). Histone H3E73Q and H4E53A mutations cause recombinogenic DNA damage. Microbial Cell 7(7): 190-198. doi: 10.15698/mic2020.07.723
© 2020 Ortega et al. This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduc-tion in any medium, provided the original author and source are acknowledged.
Abstract:
The stability and function of eukaryotic genomes is closely linked to histones and to chromatin structure. The state of the chromatin not only affects the probability of DNA to undergo damage but also DNA repair. DNA damage can result in genetic alterations and subsequent development of cancer and other genetic diseases. Here, we identified two mutations in conserved residues of histone H3 and histone H4 (H3E73Q and H4E53A) that increase recombinogenic DNA damage. Our results suggest that the accumulation of DNA damage in these histone mutants is largely independent on transcription and might arise as a consequence of problems occurring during DNA replication. This study uncovers the relevance of H3E73 and H4E53 residues in the protection of genome integrity.