A global view of substrate phosphorylation and dephosphorylation during budding yeast mitotic exit

Authors:

Sandra A. Touati1 and Frank Uhlmann1

doi: 10.15698/mic2018.08.644
Volume 5, pp. 389 to 392, published 25/07/2018.

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Affiliations:

1 Chromosome Segregation Laboratory, The Francis Crick Institute, London, UK.

Keywords: 

Cell cycle, kinases, mitosis, mitotic exit, phosphatases, phosphoproteomics

Corresponding Author(s):

Sandra A. Touati, Chromosome Segregation Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK; sandra.touati@crick.ac.uk Frank Uhlmann, Chromosome Segregation Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK; frank.uhlmann@crick.ac.uk

Conflict of interest statement:

The authors declare no conflict of interest.

Please cite this article as:

Sandra A. Touati and Frank Uhlmann (2018). A global view of substrate phosphorylation and dephosphorylation during budding yeast mitotic exit. Microbial Cell: 5(8): 389-392. doi 10.15698/mic2018.08.644

© 2018 Touati and Uhlmann. This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduction in any medium, provided the original author and source are acknowledged.

Abstract:

The cell cycle is the process by which a cell duplicates its DNA during S-phase and divides its chromosomes during M-phase, creating two genetically identical daughter cells. Cell cycle events are ordered by synthesis and degradation of key cell regulators and by phosphorylation and dephosphorylation of numerous substrates. Phosphorylation can alter the activity, interactions or subcellular localization of a protein. A substrate’s phosphorylation status is the readout of competing activities of kinases and phosphatases that target each of its phosphorylation sites. In our recent study (EMBO J. 37, e98745), we performed time-resolved global phosphoproteome analysis of a period during the cell cycle known as mitotic exit. During this time, numerous cell biological events happen in fast succession but in strict order. First, at the metaphase to anaphase transition, the mitotic spindle elongates to pull maximally condensed chromosomes to opposite cell halves. Shortly after that, spindles disassemble and chromosomes decondense, before finally cell division is completed by cytokinesis. Our time-resolved phosphoproteome analysis of this period in budding yeast provided a survey of the principles of phosphoregulation used to order these events.